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Stem rot caused by the fungus Sclerotium rolfsii is an important disease of soybean in Indonesia. This disease caused a significant yield loss averageof13-59%. This study aims to determine the ability of mycorrhizal to control stem rot disease in the two soybean cultivars.Fourdoses(0 g crop, 5 g crop, 10 g crop, and 15 g crop)of mixed mycorrhizal: Acaulosporasp, Glomus manihotis, and Gigaspora margarita (Mikofer) were applied to Anjasmoro and Kipas Merah soybean cultivars at planting time. Inoculation of S. rolfsiiwas carried out at 3 weeks after planting using a completely randomized design (CRD) with three replications. The variables observed: Incubation Period, Disease Incidence, Lesion Length, andSalicylic Acid Content. The results showed that the application of mixed mycorrhizae (micofer) was able to increase the resistance of soybean plants to stem rot disease. The percentage of affected plants decreased by 20.06% and 19.03% in the Anjasmoro and Kipas Merah cultivars respectively; the incubation period was 10.30 days and 10.44 days longer than the incubation period in non-mycorrhizal plants;the length of the lesions was shorter than the length of the lesion in the non-mycorrhizal plants. The salicylic acid content increased in both cultivars.