Studying the Biofilm Production by E. coli and Their Virulence Gene in Pregnant Women with Urinary Tractinfection

Abstract

Infection of the urinary tract (UTI) is prevalent during pregnancy and can be related to harmful effects for both the mother and the child. The elevated risk of disease in these patients was due to physiological changes, with less focus on Escherichia coli the most frequent causative factor. Our aim was to investigate the biofilm production by E. coli and Lux gene in pregnant Iraqi women with UTI. During the period extended from January to July 2020,a total of 40 from patients’ samples were collected from Baghdad Teaching Hospital diagnosis by biochemical test. The results show Out of the forty samples, 14 isolates showed a sharp band on gel electrophoresis after the amplification process by PCR which mean that those 14 isolates possess the LUX gene. Those 14 samples showed higher level of biofilm production when compared to the production of biofilm by the LUX negative isolates.