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Aquaculture is one of the main food production sectors to deal with the high demand for food due to the human population explosion. The demand for fish has increased in recent years due to population growth and the constant search for a healthy diet. The present study was conducted to assess the impact of Somatotropin hormone on Biochemical alterations such as Proteins, AST and ALT in certain tissues like Muscle, Liver and Gills of fish, Catla catla. The three different dosages of Somatotropin hormone such as 3mg, 6mg and 9mg per kg of diet was fed orally to the three experimental fish groups. The fourth group of fish were fed with diet without hormone. The biochemical alterations were recorded after 15days, 30 days and 45 days in Muscle, Liver and Gills of fish. The highest increment in tissue Protein content was observed under 9mg Somatotropin/kg diet followed by 6 mg Somatotropin/kg diet and 3 mg Somatotropin/kg diet in skeletal muscle, liver and gills of fish. Hence, it was clear that the anabolic hormones such as Somatotropin play an important role in enhancing the tissue protein content for nutritional purpose of man. Not much change was observed in AST levels of muscle, liver and gills of fish Catla catla. Significant decrease found in ALT levels of liver where as not much difference in muscle and gills.