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The experiment was conducted in the vegetable field of the Department of Horticulture and Landscape design, College of Agriculture and Forestry, University of Mosul, during the spring growing season of 2022. The aim was to study the effect of spraying with nano calcium fertilizer at three concentrations (0, 1.5, and 2.5 g/L) on some anatomical characteristics of two imported potato varieties (EL-Beida and Montreal).The nano calcium fertilizer was sprayed at three stages of plant growth: the first spray after field emergence, second and third sprays with a 20-day interval between each spray. The experiment involved 6 treatments which conducted in a split-plot system arranged within Randomized Complete Block Design, where the main plots were considered as varieties, and the sub-plot were the concentrations of nano calcium. The data were analyzed using the computer software SAS (2017), and the Duncan's multiple range test was used to test the means at a significance level of 0.05.