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Checking the phytochemical and anti - oxidant properties of the extracted oil from thrown away fish skins enables its use in the production of a superior food item. This study was conducted to extract the oil from Rohu fish skin and determine what phytochemicals it contains and how effectively it combats free radical damage (Labeo rohita). Powdered dried fish skin was diffused in n-hexane for twenty-four hours, tried to follow by evaporation at the boiling point of the solvent. Phytochemical screening was used to determine the oil's phytochemical constituents. The petroleum product includes alkaloids, glycosides, phenolics, saponins, total sugars, steroids, and coumarin, according to the results. In addition, the results revealed that the oil is devoid of active substance such as flavonoids, tannins, anthrocayanine, anthroquinone, balsams, as well as volatile oil. The antioxidant analysis was performed, and the phenolic element test, the free radical-scavenging DPPH activity test, the ABTS decolorization action test, and even the FRAP assay all indicated that the oil had a high level of antioxidant activity. According to the results, the oil has a total phenolic content of 6.49 0.01 g/mL, DPPH free radical scavenging activity of 3.84 0.02%, ABTS decolorization activity of 3.93 0.02%, and FRAP activity of 995 1.35 g/mL. This research indicates that carp fish skin oil contains phytochemicals and antioxidant activity.