Main Article Content
The current study aimed to find Antibiotic susptipility profile and virulence factors profile of staphylococcus aureus isolated from otitis media, for this purpose 200 ear swabs collected from patients suffering from otitis media whom attending to the ENT consulting clinic at Tikrit Teaching Hospital and the private outpatient clinic in Tikrit city. Bacterial isolation and identification were done , virulence factors profile detected by classical and genetic methods, and Antibiotic susptipility were done.
The result shows that out of 200 ear sample collected from otitis media, staphylococcus aureus isolated in rate of 26.1% (46 out of 200), virulence factor detection by classical methods shows that he results of the current study showed that Staphylococcus aureus possessed protease, lecithinase, and urease by 95.6%, 89.1%, and 89.1%, respectively, while the enzymes denase and hemolysin appeared by 45.6% and 71.7%, respectively. When using genetic methods, the detection of virulence genes FnbA, Etb and Sea were 80%,70% and 23% respectively. The results of the current study showed that the highest rate of resistance of staphylococci towards antibiotics was towards amoxicillin, reaching 67.3%, and the lowest towards vancomycin and azithromycin which are 9.5%