Study the Chemical Structure characterization, Antibacterial Activity and Anticancer and Cytotoxicity of Exopolysaccharides (EPS) Produced from Lactobacillus Acidophilus

Abstract

Background:Exopolysaccharides (EPSs) from bacteria are being produced in research thanks to the growing trend of utilising natural polymers in numerous fields. Bacterial EPSs are particularly interesting to the domains of science, medicine, and the food business because of their distinctive structural characteristics.

The aim:The current study focused on the Exopolysaccharides from  Lactobacillus acidophilus and study its chemical structural characterization ,antibacterial activity against  isolates as S.aureus, P. aeruginosa,E.coli, P.mirablis , E. feacalis and its anticancer and the cytotoxicity in cell lines.

The material and methods: EPS extraction from Lactobacillus acidophilus with The ethanol precipitation method and determination of carbohydrate content withthe phenol-sulfuric-acid technique, study the chemical structure with FTIR  then determination of MIC followed by the antibacterial activity  assay by agar diffusion method Finally investigate the anticancer and cytotoxity of EPS by Neutral red uptake assay  .

The results: the results of determination carbohydrates rate in purified EPSs were recorded the carbohydrate content was 80% expressed as a percentage of EPS dry weight (w/w%)  The antibacterial activity stated there was a favorable effect of EPSs against the isolates employed in the study and demonstrated inhibitory zone and recorded diverse results and significant differences at (p 0.05) between the concentrations withing the  isolate as well as with other isolates and the FTIR the chemical structural characterization of EPS by FTIR showed the analysis revealed the presence of hydroxyl, carboxyl, and amide groups.The anticancer activity reoprtedthe concentration causing 50 % proliferation inhibition of MCF7 is (IC50) by EPS from L. Acidophilus strain towards MCF-7 was 97.93  mg/mL^{- ,} in same time the cell viability at highst concentration  of EPS (200 mg/ml ) was 48.03 %  while the lowest concentration 6.25 mg/ml showed no effect on the cells  while the Cytotoxicitythe results revealed 278.8  mg/ml causing 50 % proliferation inhibition of WRL-68  is (IC50) by EPS from L. Acidophilus strain towards WRL-68 was 278.8 mg/mL^-1.The EPS was an antibacterial  that worked against both gram positive and gram negative isolates as antibacterial agent. and the chemical structural revealed that presence of hydroxyl, carboxyl, and amide which similar to other EPSs from lactic bacteria.

Conclusion: the findings showed  anticancer activity against the breast cancer cell line