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The current study aimed to find the effect of infection with Aeromonas hydrophila in some haematological and biochemical parameter in Cyprinus Carpio. For this, 70 samples of fish (50 fish suspected with Aeromonas hydrophila and 20 healthy fish) were in Babylon provenance from June-July 2021. The results of the current study showed that Aeromonas spp. detected in a rate of 88.5% according to results of culture and 80.6% of them belong to Aeromonas hydrophila according to PCR test. In addition, there is a significant decrease in the level of (red blood cells, packed cell volume and haemoglobin concentration) in the infected group compared with the healthy group, Whereas there is a significant increase in the level of white blood cells in the infected group in comparison with the healthy group, regarding biochemical tests we noted a significant decrease in the level of (total protein, albumin and globulin) in the infected group compared with a control group. We conclude from this study that infection with Aeromonas hydrophila can induce alteration in the level of blood and some biochemical parameters and causes economic losses in this field of production.