The Histopathological Effect of Verapamil Against the Nephrotoxicity of Gentamicin in Rats

Abstract

Excess amount of gentamicin is a clear cause of nephrotoxicity and the possibility of death, so this study aimed to verify the effect of gentamicin on the tissue sections of the kidneys, and treatment with verapamil to treat the effects caused by gentamicin in the kidneys of rats.

METHODS: A total of 30 rats (3 to 6 months old) raised at high ambient temperature were divided into three equal groups, 10 rats each. The first control group was administered with normal saline, the second group was treated with gentamicin (80 mg/kg body weight by intraperitoneal injection), and the third group was given gentamicin with verapamil at a concentration of 80 mg/kg for gentamicin by intraperitoneal injection at a concentration of 3 mg/kg for verapamil. By oral administration for 30 days.

The samples of the tissue sections of the kidney were taken and all the fatty tissue attached to the organ was removed, as they were placed in a package containing a solution of (10)% formalin for the purpose of histological study of the kidney organ in the study and the activities were evaluated.

Results: In the gentamicin-injected group, the glomeruli belonging to the renal granules showed degeneration and necrosis, and some of them showed dilation, bleeding and atrophy. In the other section, Bowman's area increases, with a sharp expansion of the capsular space, and hemorrhages occur in the epithelial tissue lining the proximal and distal convoluted tubules. Microscopic examination of the gentamicin and verapamil groups indicated the appearance of the kidney cortex in its normal shape with a slight splitting of the capsular space around the glomeruli and the presence of a slight thickening of Bowman's capsule with the nature of epithelial cells in the lumen of the tubules.

Conclusion: treatment with gentamycin results in significant biological changes in a manner that depends on the doses administered to the kidneys. Subsequently, a significant defect in renal tissue appears, compared to the group that included gentamycin and verapamil, where the vast difference between them and the treatment of the defect occurred.