Evaluation of Non-Pressurized Topical Spray Formulation of Miconazole and Neomycin

Abstract

Background: The prepared formulation of Non-Pressurized Topical Spray of Miconazole and Neomycin was analyzed in GC-MS depicting various components present and their biological activity. Antimicrobial and antifungal activity depicts that the maximum inhibitory zone was observed for non-pressurized topical spray in comparison to drug controls, furthermore, performed the cytotoxicity study of the formulation.

Material and Method: Gas Chromatography Mass Spectrophotometry, antibacterial, antifungal, and cytotoxicity experiments were performed on a non-pressurized topical spray formulation of Miconazole and Neomycin.

Results: The results of the Gas Chromatography Mass Spectrophotometry (GCMS-TQ 8050 NX with quadrupole) analysis show that Miconazole (9.42%) functions as an antifungal agent, Dodecanoic acid (10.6%) is as a disinfectant, Nonaneaol (0.63%) acts as an antibacterial agent, and 1H-Indole 3-acetic acid (5.90%) acts as a cell growth and organ development agent,  Propylester (14%) as an antioxidant and cancer preventative, tetradecanoic acid Oleic acid (8.31%) acts as a 5-reductase inhibitor, Allergenic, reductase inhibitor, anti-inflammatory, antiandrogenic, cancer preventative, antialopecic,  antileukotrience D4, 9-octadecenoic acid (Z)-2-hydroxy (4.05%) and Neomycin (4.71%) having antibacterial activity. Candida parapsilosis, Candida tropicalis, Candida albicans, and Candida krusei showed broad antifungal activity, with inhibition zones of 18 mm, 23 mm, 19 mm, and 22 mm for non pressurised topical spray, respectively. Inhibition zones for miconazole drug solution was 16mm, 21mm, 17mm, and 20 mm. Antibacterial activity was reported on Escherichia coli, Staphylococcus aureus, and Micrococcus luteus, with zones of inhibition of 15mm, 17mm, and 25mm for nonpresurized topical spray and 13mm, 14mm, and 22mm for Neomycin solution, respectively. Cytotoxicity tests were performed on HACAT (Human Epidermal Keratinocyte Line) at concentrations of 6.25, 12.5, 25, 50, 100, and 200 micrograms per millilitre to determine percent inhibition after 24 hours.

Conclusiuon: The non-pressurized topical spray’s chemical constituents act as antifungal agent, disinfectant, antibacterial agent, cell growth development agent, cancer preventative agent. Spray showed broad antifungal and antibacterial activity against Candida parapsilosis, Candida tropicalis, Candida albicans, Candida krusei, Escherichia coli, Staphylococcus aureus, and Micrococcus luteus.  Because the inhibition was less than 50%, non-pressurized topical sprays of miconazole and neomycin were determined to be non-toxic or had no appreciable cytotoxicity.