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This study was prepared to find out the effect of downer cow syndrome in local cows on some biochemical parameters, 50 blood samples were collected from separate areas of the city of Kirkuk (40 blood samples from animals infected with resting cows and 10 blood samples from healthy animals) at the age of 3-8 years. Each of the parameters (Ca, Mg, P, Total protein, Albumin, Globulin, Glucose, ALT, AST, ALP, CPK) were studied. It was noted that there was a significant decrease (p<0.05) in the values of (Ca, Mg, P, Total portion, Albumin, Globulin, Glucose) in the infected cows compared with the healthy cows, while there was a significant increase (p<0.05) in the values of (ALT, AST, ALP, CPK) in cows infected with downer cow syndrome compared to healthy cows. We conclude from this study that downer cow syndrome has an adverse effect on some biochemical parameters, and these parameters in turn give an indication of infection and ways to control it through good management and treatment programs.