Green Synthesis of Silver Nanoparticles Using Abutilon Grandifolium Aqueous Leaf Extract, Characterization and Evaluation of their Anti-Bacterial, Anti-Oxidant and Anti-Inflammatory Properties

Abstract

Developing dependable and environmentally friendly methods for the synthesis of nanoparticles is a critical step in the field of nanotechnology. Silver nanoparticles (AgNPs) are significant due to its superior chemical, physical and biological properties, as well as their numerous applications. The present article reports an environmentally benign method for synthesizing AgNPs using the aqueous leaf extract of the plant Abutilon grandifolium, their characterization and evaluation of its anti-bacterial, anti-oxidant and anti-inflammatory activities. The synthesized nanoparticles were characterized by Ultraviolet-Visible (UV-Vis) spectroscopy, which shows an absorption peak at 415 nm due to the Surface Plasmon Resonance (SPR) property. The Energy Dispersive X-Ray (EDAX) spectroscopy shows that the synthesized AgNPs obtained were pure in form. The zeta potential value was found at -36.4 mV. High-Resolution Transmission Electron Microscopy (HR-TEM) showed that the nanoparticles were spherical. The synthesized nanoparticles were checked for the anti-bacterial activity of various gram-positive and gram-negative bacteria. The results obtained show perfect activity against Bacillus subtilus. DPPH assay and albumin denaturation assay determined the anti-oxidant and anti-inflammatory activity. The results show that the synthesized AgNPs possess excellent anti-oxidant and anti-inflammatory activities.