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Interest in green nanotechnology in nanoparticle biosynthesis is growing among researchers. Nanotechnologies due to their physicochemical and biological properties have applications in diverse fields, including drug delivery, sensors, optoelectronics and magnetic devices. Green synthesis of nanoparticles is an eco-friendly approach, which should be further explored for the potential of different plants to synthesize nanoparticles. The sizes of AgNPs are in the range of 1 to 100nm. Multi drug resistance has become an emerging and challenging issue for pharmaceutical industry as more and more bacteria are developing drug resistance towards many antibiotics, also over use of these synthetic drugs causes toxicity leading to further detrimental effects on human body. This study focused on the synthesis of silver nanoparticles, which is necessary for safe and effective exploitation of silver nanoparticles in collaboration with plant derived metabolites as a substitute for harmful synthetic drugs. Silver nanoparticles were synthesized using Bauhinia variegata; synthesized silver nanoparticles were then subjected to check antibacterial activity against multidrug resistant bacteria like Staphylococcus aureus and Klebsiella pneumonia by well diffusion method.AgNPs were synthesized by using 1mM AgNO3 solution mixed with hydroalcoholic extract of Bauhinia variegata. The characterization of the prepared AgNPs was done by UV-Vis spectrometry and FTIR spectroscopy. The characterization results of the prepared AgNPs displayed that the silver nanoparticles are formed and stabilized by plant phyto-constituents and also exhibited virtuous antimicrobial property. Green synthesis process is a pivotal area in nanotechnology and usage of natural resources is the best choice for the making of NPs as a sustainable, eco-friendly, inexpensive and free of chemical contaminant method. These AgNPs have several potential biological and medical applications.