Pathological and Immunohistochemical Characteristics of the Pancreas in Infants who died from ARVI

Abstract

purpose of the article was to make immunomorphological analysis of the pancreas endocrine apparatus structure in infants under one year old died from viral infections.

Material and methods. The results of 39 autopsies of children under one year of age who died from acute respiratory viral infection.

The work used case histories and autopsy results. Systemic morphological examination of organs was performed. Immunoperoxidase method for B cells determination (monoclonal antiserum NCL-JOVI 1, clone JOVI 1; working dilution 1: 100), A-cells (monoclonal antiserum NCL-DFB1, clone DF-B1; working dilution 1: 100),G cells (NCL-MCTRYP monoclonal antiserum, clone AA1; working dilution 1: 100) and serotonin (NCL-LN5 monoclonal antiserum, clone LN-5; working dilution 1:40) was used in the study.

Results and discussion. It was found that in viral infection, the lesion was of toxic and dyscirculatory nature and manifested mainly in plethora of the organ, laxity or sclerotic gland thickening and lymphohistiocytic infiltration, atrophy and dysplasia of endocrine parenchyma cells. Accumulation of a pinkish, amorphous type of secretion was observed in the lumen of most of the ducts. Inside the islet apparatus numerous cellular elements are located non-compactly.Voids and cell death in the pancreatic islets of Langerhans, an increase in the number of glucagon-producing cells, a decrease in the number of insulin-synthesizing cells and a lack of serotonin were found between the elements.