Eefective Callus Formation and Plant Regeneration of Chromium Induced Mutants in Eleusine Coracana(L.) Gaertn.

Abstract

In the present work two accessions of Eleusinecoracana(E1, E2) were tested for callus formation and plant regeneration. The seedlings were raised in 10 different molar concentrations of Cr (10^-1 to 10^-10M K₂Cr₂O₇). For dedifferentiation various seedling parts like hypocotyl and cotyledons or whole seedlings were used. Three growth hormones (auxin 2, 4-D and cytokinins, kinetin and BAP) were used for callus induction. 2, 4-D was used in combination with kinetin (KN) and BAP in ratios 3:1, 4:1 and 5:1. The callus was observed on the 5^th day from inoculation. The callus formation was found in all treatments except 10^-2M and 10^-3M Cr concentration. The callus formation was observed maximum in MS with 2, 4 –D and BAP in 5:1 in both the accessions. The callus was white brownish in colour and granular in nature. The MS medium with 2, 4-D and kinetin didn’t respond. In E1, 10^-10M and 10^-6M showed better and in E2 10^-8M and 10^-6M showed good callus formation.