Determine the frequency of Bacterial Pathogens in Neonatal Sepsis and their Sensitivity to Different Antibiotics.

Abstract

Neonatal sepsis is a blood infection that occurs in an infant younger than 90 days old. Earl onset sepsis is seen in the first week of life. Late onset sepsis occurs after 1 week through 3 months of age. The aim of the present research to evaluate the Prevalence rate of Bacterial pathogens in neonatal sepsis and to determine the sensitivity of these pathogens to commonly used antibiotics at Department of Pediatrics, at PIMS Hospital Islamabad Pakistan from January 2016 to December 2016.in the current study 100 neonates were enrolled and Sample for Blood culture was taken under aseptic measures by standardized techniques and were saved in culture bottle. It was collected and transported to the Hospital laboratory by single person and inoculates & incubated there. The growth of the pathogen was observed for next 5 days and sensitivity pattern was checked by using different antibiotic discs. Culture and sensitivity pattern as shown by blood culture report was noted. The result show that the Frequency of bacterial pathogens were 35(34%) Klebsiella, 28(26%) Pseudomonas, 22(20%) E.coli and Staphylococcus 26(24%), while frequency of antimicrobial sensitivity reveals 77% Ciprofloxacin, 83% Amikacin, 19% Penicillin and 12% Gentamicin for Staphylococcus Aureus. Sensitivity for Pseudomonas was 58% Ciprofloxacin, 61% Amikacin, 1% Penicillin, Imipenum (81%) and 37% Gentamicin. For pathogens Klebsiella it was 47% Ciprofloxacin, 37% Amikacin, and 9% Impineum while Ciprofloxacin 49%, Amikacin 54% and Imipenum 78% for E.coli. From The Present Research we concluded that Klebsiella and Pseudomonas are the leading pathogens in neonatal sepsis and the most commonly used antibiotics using in our setup are highly resistant with them which may be used when culture reports are awaiting.