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The aim of the present study is to identify the important functional groups and Phytochemical constituents of the ethanol extract of Eugenia jambolana, which was analyzed by GC.The GC-MS analysis of the ethanol extract from the seed part of Eugenia jambolana detected the presence of 17 different compounds in each extract. Both extracts exhibit different compounds and biological activity. Results revealed various major compounds among which the Phytochemical compounds were identified: Hexadecanoic acid, Dotriacontane, Oleic acid, Cyclohexane, 1-(1,5-Dimethylhexyl)-4-(4-Methylpentyl), Hexadecanoic acid, Ethyl ester, T-Butyl Cyclopentaneperoxycarboxylate, 2,3-Anhydro-D-Mannosan, 2,3-Anhydro-D-Galactosan, Alpha-Cadinol, Tau-Murol, Diglycerol.GC-MS analysis revealed that the extracts contain a high concentration of phytoconstituents, which may be bioactive components involved in the plant's therapeutic properties..GC-MS analysis revealed that the extracts contain a high concentration of phytoconstituents, which may be bioactive components involved in the plant's therapeutic properties. These results can be used as a good preliminary indication for future applications of antifungal, antibacterial, antimalarial, antidiabetic, anti-inflammatory, antimicrobial, preservative, and antioxidant.