Evaluated the Antibacterial Activity and Cytotoxicity of Wound Dressing for PCL/Chitosan and PCL/ Hydroxyapatite Nanofiber

Abstract

The development of novel methodologies for completely functioning skin tissue regeneration was necessary to improve the healing quality of damaged lesions. Electrospin nanofibrous scaffolds based on Poly (ε-caprolactone) modified with Chitosan and hydroxyapatite biomimetic nanofibrous scaffolds at 0.01wt% and 0.4 percent were investigated in this research. A disc diffusion test was used to assess antibacterial activity against Escherichia coli (E.coli) and Staphylococcus aurous (S.aureus). The cytotoxicity of the MTT cell line was assessed using an extraction technique, and proliferation was detected.

Antibacterial studies have shown that PCL / Chitosan and PCL / HA nanofiber membranes containing 4% gentamicin on bacterial cultured agar plates significantly inhibit the growth of adjacent E. coli and S. epidermises. Bacteria are possible, indicating
that antibacterial activity was classified as "strong" or "significant" against the two types of bacteria.Compared to pure PCL, cell viability results show higher activity and improved mineralization, allowing PCL nanofiber films as a matrix of cell proliferation
with minimal damage to cells. Shows sex and can be used in biomedical applications.