Ethno Pharmacological Screening of Selected Compounds from Crataegus Rhipidophylla Plants Grown at Swat, Pakistan

Abstract

The present study involves the photochemical screening, mineral analysis and biological activities of crategus rhipidophylaa. Crataegus rhipidophylla collected from district Swat. It is medicinal plant. We used solvent methanol, chloroform, aqueous extract of whole plant of crategus rhipidophylla. The whole parts of the selected medicinal plant were washed, shade dried and then powdered for qualitative analysis. Aqueous, methanol and n-hexane extracts of whole plant were used to find out the photochemical constituents, mineral analysis, antioxidant and antibacterial activity in the crataegus rhipidophylla. The results of the phytochemical analysis of crategus rhipidophylla showed that the terpenoids, tannin, flavonoids, glycosides, alkaloids, saponins were present in all extracts except of rare phytochemicals. Mineral analysis showed that the plant extract contain copper (0.063mg/L), iron (mgL62.26/), manganese (0.448mg/L), lead (0.627mg/L), zinc (0.757mgL/), cadmium (0.211mg/L), chromium (1.332mg/L), cobalt (0.419mgL/). Antibacterail activity was carried out against five different bacterial strains: Esheria coli, Pseudomonas Aeruoginosa, Salmonella typhae, Shigella, Staphylococcus aureus. The antibacterial activity was determined by agar method. Antioxidant activity was carried out by DPPH method. The highest antibacterial activity; 15±0.99 mm in Hexane extract against S. aereus and 15±0.85 mm in methanol were obtained against Samonellatyphae respectively. The highest antioxidant activity was observed at 80ppm n-hexane (86.65) second highest activity was observed at 60ppm n-hexane(71.98)  methanol was present at 80ppm (71.87) it was also observed that this plant is potential source of antioxidant agent and the sample has good quantity of phytochemicals. The current investigation will help the researcher as basic information for future research in manipulating the buried potential of this important plant which has not been explored so far.