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The development of antibiotic resistance in pathogenic bacteria has been a problem. Hospitals are where the probability of developing multidrug-resistant (MDR) bacteria is much higher. We investigated the presence of MDR bacteria in tap water collected from Hayatabad Medical Complex and cantonment board hospital, Peshawar. Bacterial strains were isolated from tap water, and their antibiotic sensitivity profile was investigated. Six commonly used antibiotics; Co-trimoxazole, gentamycin, clindamycin, augmentin, erythromycin, and ciprofloxacin. Two bacterial strains were isolated from the tap water and identified as Staphylococcus aureus and Micrococcus luteus. Both the bacterial strains were found resistant to clindamycin and augmentin antibiotics. Micrococcus luteus also showed resistance to clindamycin while sensitive to the rest of the antibiotics used during the experiment. The antibiotics gentamycin, erythromycin, and ciprofloxacin were highly effective against the isolated bacterial strains. Further investigation is suggested to find how these bacteria reach hospitals' sources and storage tanks. It is also indicated that tap water from other hospitals should be investigated for MDR bacteria.