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Local people have used medicinal plants for generations in different areas. Unfortunately, many modern and even local communities don't have adequate awareness about the importance or value of medicinal plants. For that reason, this study was considered to survey and explore the uses of medicinal plants consumption based on their antimicrobial properties. For this purpose, Rhamnusvirgata is investigated based on scientific references. Antimicrobial activity was investigated in the bark of Rhamnusvirgata. The dried bark of Rhamnusvirgata was extracted using various solvents, including chloroform, Ethyl acetate and Deionized water. The whole work demonstrates that it serves as a potential antimicrobial crude drug and a source for natural compounds that act as new antibacterial and antifungal agents. It was effective against Salmonella typhi, Shigella, Staphylococcus aureus, Pseudomonas aeruginosa, and E. coli. Fungi showed little activity. The result indicates that Rhamnusvirgata is the best source of antimicrobial activity.