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A case study of 72-yearmale with overweight, diabetic and hypertensive condition, presented with gout, frequent gastric complications. After studying the symptoms, morphology, patient condition, blood test, the patient was doubtful for the presence of B-CLL and immunophenotyping and FISH (Fluorescence in-situ Hybridization) analysis was done to confirm the same. After the immunophenotyping the patient was confirmed for the B-CLL leukemia and thus was precededforward for further treatment therapies. In the present study our main focus is to determine the importance of immunophenotyping using the flowcytometry for determining the disease and be specific in the process of giving the specified treatment to the affected individual.