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Essential oil isolated from the aerial parts of Clematis cirrhosa L., growing in Algeria, was evaluated for its chemical composition and antioxidant activities for the first time, this species is used in Algerian folk medicine. The essential oil obtained by hydrodistillation was analyzed by GC/MS; the results indicated that the OE of C. cirrhosa contained 12 compounds, of which three predominant compounds are: Phytol (30.1%), Palmitic acid (29%), Juniper camphor (14.5%). The antioxidant activities were tested by using several methods (TAC, DPPH·, ABTS·+, FRAP and CUPRAC), the OE showed a high antioxidant activity in TAC, FRAP and ABTS tests with (291.36 ± 1.82 mg AAE/g, 119.71 ± 2.24 mg TE/g, 128.91± 0.40 µg TE/mg) respectively. Whereas a moderate antioxidant activity was obtained in CUPRAC and DPPH with (IC50 =5.10 ± 0.14 mg/ml). From our results the antioxidant activity of EO C. cirrhosa was certainly due to its chemical composition.