Healing Efficiency of Zinc Oxide Nanoparticles in Various Concentrations on the Full Thickness Wounds in Rabbits (Oryctolagus Cuniculus)

Abstract

Integumentary system is the basic system of the body that acts as the first line of defense against the environment. Any type of attack result in the damage of skin known as wound, depending on nature and magnitude of attack. For proper functions of skin healing of wound is compulsory and depends on many factors. Many natural and synthetic products are responsible for quick healing of clinical wounds. Zinc is one the major agent appropriate for this purpose especially when topically used in Nano Zinc oxide form. This study was carried out to find out the healing capacity of Nano Zinc Oxide ointment on surgical wounds in various concentrations as 0.25, 0.5, 0.75 and 1%. For this purpose 10 healthy mature rabbits were selected, as the ten experimental models. Four surgical wounds were developed on lateral side of each rabbit and each wound was treated with one of four different concentrations of ZO NPs as 0.25, 0.5, 0.75 and 1% respectively.   The healing efficiency of wounds and ointment was measured on days 5, 10, 15, 20 and 25^th through healing time, tissues tensile strength, histopathology, and contraction rate of the wound by randomized recording method. Obtained data was analyzed with the help of ANOVA and results showed that wound healing time was the minimum as 16^th day for 1.00% and maximum 21^st day for 0.25% concentration of NPs ZO. Same is the case for rate of wound contraction and histopathology that results are in support of 1.00% concentration and not encouraging for 0.25%. This study appeals for the use of ZO NPs ointment for the healing of surgical wounds but more study of ZO NPs in the field of surgery at more molecular level is need of the day.