Synergistic Anti-Diabetic Activity of A Polyherbal Extract in Streptozotocin Induced Diabetic Rats

Abstract

The aim of the present study was to compare the anti-diabetic activity of a polyherbal extract in streptozotocin induced diabetic rats. The ethanolic extracts of leaves of Alstonia scholaris, Centella asiatica, Corchorus trilocularis and Morinda pubescens were prepared. The wistar albino rats were injected with 60 mg/kg of streptozotocin intraperitoneally to induce diabetes. After six weeks of streptozotocin injection, individual extract and polyherbal extract were daily administered at a dose of 200 mg/kg body weight to diabetic rats for a period of 21 days. Blood samples were collected from the retro orbital plexus of the eye and blood glucose level was estimated by glucose oxidase-peroxidase method. The study indicated that polyherbal extract at a dose of 200 mg/kg body weight showed significant decline in blood glucose level as compared to individual extract.The elevated level of SGPT, SGOT in the diabetic controlled group reflected the significant alteration of liver function by STZ induction and was found to be equipotent to Glipizide in restoration of the elevated enzyme levels to normal. The elevated lipid levels (triglyceride and total cholesterol) were restored to near normal by extract for all the estimated parameters. The results of the PHE on treated group were found to restore the significant glycemic level to the near normal level thereby indicating antihyperglycemic activity.