THE COMPARISON OF HIND LIMB MASS LOSS IN HOUSE MICE PRESERVED WITH ADVANCED FIXATIVE SOLUTIONS OF ETHANOL-GLYCERINE AND 4% FORMALDEHYDE

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Ria Margiana, Amanda Natalie Wijaya, Sasanthy Kusumaningtyas, Deswaty Furqonita, Rahmadini

Abstract

Cadaver preservation usually uses formalin as a fixative solution. However, formalin has many negative effects on health and the environment. To reduce these negative effects, an alternative fixative is needed to be explored. Ethanol-glycerin (EG) can potentially be an alternative to formaldehyde as a fixative solution. Therefore, this study aims to compare the hind limb mass loss in mice treated with advanced preservation of EG and 4% formalin. This research method used adult male house mice (Mus musculus) aged 3-6 months with body weight ranging from 20-30 grams. The research group consisted of the control group using 10% of formalin, the EG group using ethanol-glycerin, and the formalin 4% group using 4% of formalin as a fixative solution. Hind limb mass and total were observed after 6 weeks post burial. The results showed a hind limb mass loss after 6 weeks for each group. Hind limb The hind mass loss in the EG fixative group had a significant mass loss compared to the 4% formalin fixative group on a weekly basis.

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How to Cite
Ria Margiana, Amanda Natalie Wijaya, Sasanthy Kusumaningtyas, Deswaty Furqonita, Rahmadini. (2021). THE COMPARISON OF HIND LIMB MASS LOSS IN HOUSE MICE PRESERVED WITH ADVANCED FIXATIVE SOLUTIONS OF ETHANOL-GLYCERINE AND 4% FORMALDEHYDE. Annals of the Romanian Society for Cell Biology, 2677 - 2682. Retrieved from http://annalsofrscb.ro/index.php/journal/article/view/378
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