Isolation and Characterization of Patulin Mycotoxin from strawberry fruits

Abstract

pencillium expansum is the  most common fungi isolated  from strawberry furits. The frequency and visibility  of pencillium expansum is (40 and 86) respectively,on Sabouraud dextrose agar.

Patulin is extracted from the penicillin expansum that grows in the strawberry fruit. Concentration of patulin in the Strawberry fruits is (14.158-24.843) µg/ml

HPLC results show the  retention time of major peak of patulin standard toxin  located at 5.098 minutes, while a filter extract of p.expansum from (5.095) which is located  in the same area .

The UV-VIS spectroscopy results of p.expansum and the standard  patulin toxin, the major peak of standard patulin toxin is located with wavelength 276 nm and 2 absorbance; while  the extract of           p. expansum which is producing  patulin toxin it located at a wavelength of 277 nm and 2.3 A.