Study of Some Virulence Factors of Bacteria Isolated from Urine among the Patients of Type 2 Diabetes Mellitus in Baghdad

Abstract

      The study included 110 urine samples from patients with type 2 diabetes mellitus from patients attending the Specialized Center for Endocrinology and Diabetes and Al-Nu'man Hospital at period from 9/1/2020 to 10/11/2020. 75 bacterial isolates were obtained and distributed as follows, 20(26.6%) isolates of E. coli, 20(26.6%) isolates of S. aureus, 15(20%) isolates of Klebsiella pneumonia. while, Pseudomonas aeroginosa, Protues mirabilis, Staphylococcus warneri and Micrococcus spp. reported 5(6.7%) isolates for each species and there were high significant (P<0.01) differences between the percentages of bacterial isolates. Samples were cultured on MaConkey and blood agar for initial detection of bacteria, and then the biochemical tests were performed on all isolates. In addition, using the API 20 E diagnostic system to diagnose the Gram-negative bacteria and API Staph to diagnose Gram-negative bacteria. Some virulence factors produced from bacterial species, including Hemagglutination, were also investigated. The results showed that the highest rate of agglutination in bacterial isolates of K. pneumonia and E. coli was 100% and 90%, respectively, while the percentages of the remaining isolates under study ranged from 20. -60%.