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Multidrug resistance Pseudomonas aeruginosa (MDRPA) is most important issue in healthcare setting. Pyocyanin production among MDRPA must increase the worseness of infection by side adverse like tissue damage subsequent harmful effects of pyocyanin for host. The killing of bacteria accomplished by inhibition of pyocyanin production may represent a promise treatment approach. The study include investigation of 50 isolates of MDRPA for pyocyanin biosynthesis and resistance for antibiotics. Molecular diagnosis using P. aeruginosa specific primer pairs, investigation of phzA, phzM and phzS using specific primer pairs by PCR were also performed. The results revealed high resistance to beta lactam antibiotics (78% for ceftazidime, 78% for cefepime and 46% for piperacillin) can indicate possessing of isolates for beta lactamases and this confirmed by dropping resistance to piperacillin to 16% when combined with tazobactam. Also the results shown the ability of MDRPA for pyocyanin production using the system of genes (phzA, phzM, phzS) among all isolates except (3 isolates for phzS). The current study conclude that all isolated of P. aeruginosa were highly virulent due to their possessing of pyocyanin biosynthesis system and beta lactamases make using of piperacillin-tazobactam and meropenem a good choice to kill bacteria along with impairment of pyocyanin production reducing the possible harmful effects of this pigment.