The Role of Some Promising Biomarkers in Diagnosis Neonatal Sepsis Caused by Multidrug Resistance Acinetobacterbaumannii

Abstract

    A total 120 newborn were studied from Neonatal Care Unit / Children Welfare Teaching Hospital /Medical city / Baghdad / Iraq ,  from November  2019 to end of February 2020, Male were 77 (64.17%) and  43 ( 35.83% ) female, age ranged between 1 day  to  27 days. 5 ml of Blood specimen collected from patients , 2.5 – 3.0 ml  inject immediately in Brain heart infusion and incubated 24 hours at 37°C  before cultured on different media (MacConkey agar and Blood agar) , after the growth of bacteria, the isolates were identified by microscopic examination , biochemical tests , Analytical profile index (API 20E) and VITEK -2 system . Another specimen (1 ml) were taken for screening level of  Neutrophil Cluster of differentiation  (nCD64) , by ELISA technique, ELISA technique was used to detect the level of expression of nCD64. The result of present study revealed the level of nCD64 in neonatal sepsis (A. baumannii+ve) group (28.86 ± 1.84 ) was significantly higher than  neonatal sepsis (A. baumannii-ve)  group (14.76 ± 2.16) and control (2.33 ± 0.58) (P≤0.01).