Evaluation of Composition and Architecture of the Platelet Rich Fibrin: An Observational Study

Abstract

Aim: To undertake a detailed examination of the cell composition and structure of Choukroun’s platelet rich fibrin using hematologic counts, scanning electron microscope and light microscopy. Additionally the aim is to also find any differences in:

• Structure and morphology when PRF is produced using two varied kinds of collection tubes, namely, glass and plastic tubes.


• Structure and morphology when two different methods of compression of PRF clot are used to get a membrane (compressed/soft)

Materials and Methods: Sample set of 14 healthy subjects participated in this study. Blood collected from each of the subjects was subjected to centrifugation; the platelet rich clot/membrane obtained was processed and subjected to light microscopy and scanning electron microscopy examination. The residual plasma was subjected to blood analysis.

Results: The study revealed that the PRF clot contained most of the platelets and more than 50% of the leukocytes from the blood sample and their distribution adhered to a distinctive three-dimensional pattern. No significant difference in the PRF architecture and composition was found between groups using different tested collection tubes and compression techniques.

Conclusion:The PRF contained most of the platelets and the leukocytes from a blood harvest into a single autologous fibrin biomaterial.