Comparison of the Effect of Adding Methionine, Synthetic and Herbal Lysine, And L-Carnitine in the Diet on the Physiological and Histological Characteristics of Laying Hens( ISA Brown)

Abstract

This experiment was conducted in the poultry field affiliated to the Department of Animal Production / College of Agriculture / University of Kirkuk, from the period 5/2/2020 until 4/29/2020 (for a period of 12 weeks), and fed for an introductory period of two weeks with experimental treatments in order to accustom the birds to the diets Experience. The aim of the study is to compare the effect of adding methionine, industrial and herbal lysine, and L-carnitine in the diet on some of the physiological and histological characteristics of laying hens. In the experiment, 200 laying hens of (Brown ISA) strain were used at the age of 40 weeks. and the birds were distributed among ten Treatments randomly by five replicates for each treatment and one replicator included four chickens, and the (experimental) nutritional treatments were ,T1: basal diet supplemented with synthetic methionine and synthetic lysine to complete the nutritional requirements, T2: basal diet supplemented with Herbal methionine and Herbal lysine to complete the nutritional requirements, T3: basal diet supplemented with 15% above nutritional requirements from synthetic methionine and synthetic lysine, T4: basal diet supplemented with 15% above nutritional requirements from Herbal methionine and Herbal lysine, T5: T1diet supplemented with  750 mg of L-carnitine per kilogram me of diet, T6: T2diet supplemented with  750 mg of L-carnitine per kilogram me of diet, T7: T1diet supplemented 1gm of L-carnitine per kilogram me of diet, T8: T2diet supplemented 1gm of L-carnitine per kilogram me of diet, T9: T1diet supplemented 1.25gm of L-carnitine per kilogram me of diet, T10: T2diet supplemented 1.25gm of L-carnitine per kilogram me of diet. The water and feed ad libitum  were available for the birds, The management conditions with as ISA Brown guide.  The statistical analysis results obtained: The biochemical characteristics of serum, which included (uric acid, glucose, cholesterol, total protein, high-density lipoprotein (HDL), low-density lipoprotein (LDL)) and enzymes (AST), aspartate aminotransferase (ALT), alanine aminotransferase, creatine kinase (ALT) (CK) creatine kinase, the antioxidant glutathione (GSH) Glutathione), there were no significant differences between the rates of all treated birds' blood serum for biochemical characteristics. In addition, there was an increase in the rate of villi height and cache depth of the intestines of fourth treatment birds significantly (P≤0.05) relative to villi in the intestines of first, third, fifth, seventh and ninth treatment birds.